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A bacterial artificial chromosome-based physical map of

Yuhua FU,Zhiqiang XIA,Shujuan WANG,Xin CHEN,Cheng LU,Mingcheng LUO,Hongbin ZHANG,Wenquan WANG

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 4,   Pages 321-329 doi: 10.15302/J-FASE-2016124

Abstract: Two new bacterial artificial chromosome (BAC) libraries were constructed for W14 ( ssp. ), a wild ancestorThe RI library has 29952 clones with an average insert size of 115 kb, while the dIII library consistssingletons, representing approximately 762 Mb of the cassava genome. 5000 clones located at the ends of BACThis BAC-based physical map provides valuable tools to understand the genetics and evolution of cassava

Keywords： cassava     BAC library     physical map    

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Development of pretreatment protocol for DNA extraction from biofilm attached to biologic activated carbon (BAC

Shuting ZHANG, Bo WEI, Xin YU, Bing LIU, Zhuoying WU, Li GU

Frontiers of Environmental Science & Engineering 2010, Volume 4, Issue 4,   Pages 459-465 doi: 10.1007/s11783-010-0249-3

Abstract: The biologic activated carbon (BAC) process is widely used in drinking water treatments.However, the bottleneck of deoxyribonucleic acid (DNA) extraction from BAC attached biofilm has to beconventional procedure was unsuccessful due to firm biomass attachment and adsorption capacity of the BACThis protocol was further tested using the vertical BAC samples from the full-scale biofilter of PinghuThe results showed the DNA yielded a range of 40 μg·g BAC (dry weight) to over 100 μg·g BAC (dry weight

Keywords： bacterial DNA extraction     biological activated carbon (BAC)     biofilm     water treatment     pretreatment protocol    

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Low-cost green building practice in China: Library of Shandong Transportation College

Yingxin ZHU, Borong LIN, Bin YUAN,

Frontiers in Energy 2010, Volume 4, Issue 1,   Pages 100-105 doi: 10.1007/s11708-009-0072-3

Abstract: This paper introduces the design idea and technique utilized in the Library of Shandong TransportationAfter reconstruction, it was changed into the construction site of the library with an area of 7000

Keywords： green building     low cost technique     energy efficiency     integrated strategy    

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software for land use regression modeling the spatial distribution of air pollutants using GDAL/OGR library

Xuying Ma, Ian Longley, Jennifer Salmond, Jay Gao

Frontiers of Environmental Science & Engineering 2020, Volume 14, Issue 3, doi: 10.1007/s11783-020-1221-5

Abstract: PyLUR comprises four modules for developing and applying a LUR model. It considers both conventional and novel potential predictor variables. GDAL/OGR libraries are used to do spatial analysis in the modeling and prediction. Developed on Python platform, PyLUR is rather efficient in data processing. Land use regression (LUR) models have been widely used in air pollution modeling. This regression-based approach estimates the ambient pollutant concentrations at un-sampled points of interest by considering the relationship between ambient concentrations and several predictor variables selected from the surrounding environment. Although conceptually quite simple, its successful implementation requires detailed knowledge of the area, expertise in GIS, statistics, and programming skills, which makes this modeling approach relatively inaccessible to novice users. In this contribution, we present a LUR modeling and pollution-mapping software named PyLUR. It uses GDAL/OGR libraries based on the Python platform and can build a LUR model and generate pollutant concentration maps efficiently. This self-developed software comprises four modules: a potential predictor variable generation module, a regression modeling module, a model validation module, and a prediction and mapping module. The performance of the newly developed PyLUR is compared to an existing LUR modeling software called RLUR (with similar functions implemented on R language platform) in terms of model accuracy, processing efficiency and software stability. The results show that PyLUR out-performs RLUR for modeling in the Bradford and Auckland case studies examined. Furthermore, PyLUR is much more efficient in data processing and it has a capability to handle detailed GIS input data.

Keywords： LUR     Air pollution modelling     GIS spatial analysis     GDAL/OGR Python     Pollutant concentration mapping    

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Near-infrared benzodiazoles as small molecule environmentally-sensitive fluorophores

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 1,   Pages 128-135 doi: 10.1007/s11705-021-2080-8

Abstract: In this work, we have optimised a general and straightforward synthetic approach to prepare a small library

Keywords： fluorescence     probes     iron     screening     library    

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Intelligent Model Library for Nonferrous Metal Industry: Construction Method and Application

Yang Chunhua, Liu Yishun, Huang Keke, Sun Bei, Li Yonggang, Chen Xiaofang, Gui Weihua

Strategic Study of CAE 2022, Volume 24, Issue 4,   Pages 188-201 doi: 10.15302/J-SSCAE-2022.04.013

Abstract: Nonferrous metals industry intelligent model library architecture based on IIoT.The target environment can parse the PMML library to load the meta-model.Nonferrous metallurgy meta-model ontology library.Nonferrous metal industry intelligent model library.Currently, 1251 meta-models are available from this library.

Keywords： nonferrous metal industry     industrial software     meta-model     intelligent model library     industrial Internet    

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Construction, characterization and application of a genome-wide promoter library in

Ting Yuan,Yakun Guo,Junkai Dong,Tianyi Li,Tong Zhou,Kaiwen Sun,Mei Zhang,Qingyu Wu,Zhen Xie,Yizhi Cai,Limin Cao,Junbiao Dai

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 107-116 doi: 10.1007/s11705-017-1621-7

Abstract: Here we report the construction of a genome-wide promoter library, in which each native promoter inclassified the yeast strains based on YFP fluorescence intensity and arbitrarily divided the entire libraryTogether, this library could provide a quick solution to identify and utilize desired promoters under

Keywords： synthetic biology     yeast     promoter activity     metabolic engineering     xylose utilization    

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Construction of a universal recombinant expression vector that regulates the expression of human lysozyme in milk

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 3,   Pages 382-389 doi: 10.15302/J-FASE-2018211

Abstract: methodology is expected to have the advantages of both plasmid and bacterial artificial chromosome (BACThe BAC of mouse whey acidic protein gene (mWAP) was modified twice by homologous recombination

Keywords： BAC recombinant methods     gene expression     human lysozyme     transgenic mice     milk expression vector    

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Impact of preozonation on the bioactivity and biodiversity of subsequent biofilters under low temperature conditions—A pilot study

Jiaxuan YANG, Jun MA, Dan SONG, Xuedong ZHAI, Xiujuan KONG

Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 4, doi: 10.1007/s11783-016-0844-z

Abstract: The combination of preozonation and subsequent biological granular activated carbon (O /BAC) filtrationThe elevated functionality in O /BAC filters can be a result of increased microbial activity, which was

Keywords： Preozonation     BAC filtration     High ammonia level     Bacterial activity     Microbial community structure     Pilot    

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High-Affinity Decoy PD-1 Mutant Screened from an Epitope-Specific Cell Library Article

Hao Liu, Chunxia Qiao, Naijing Hu, Zhihong Wang, Jing Wang, Jiannan Feng, Beifen Shen, Yuanfang Ma, Longlong Luo

Engineering 2021, Volume 7, Issue 11,   Pages 1557-1565 doi: 10.1016/j.eng.2020.11.011

Abstract: ., PD-L1 and PD-L2) or antibody (Opdivo), a small-content, epitope-oriented mammalian cell library was

Keywords： Decoy PD-1     PD-L1     Mammalian cell library     Epitope-oriented    

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Evaluate HAA removal in biologically active carbon filters using the ICR database

Hsin-hsin TUNG, Yuefeng F. XIE

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 4,   Pages 489-496 doi: 10.1007/s11783-011-0312-8

Abstract: The effects of biologically active carbon (BAC) filtration on haloacetic acid (HAA) levels in plant effluents

Keywords： biologically active carbon (BAC)     disinfection byproduct (DBP)     granular activated carbon (GAC)     haloacetic    

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Analytical solutions of three-dimensional contaminant transport in uniform flow field in porous media: A library

Hongtao WANG, Huayong WU

Frontiers of Environmental Science & Engineering 2009, Volume 3, Issue 1,   Pages 112-128 doi: 10.1007/s11783-008-0067-z

Abstract: The purpose of this study is to present a library of analytical solutions for the three-dimensional contaminantThe library is constructed using Green's function method (GFM) in combination with available solutionsThe library covers a wide range of solutions for various conditions.The library is widely applicable to solve contaminant transport problems of one- or multiple- dimensions

Keywords： solution library     contaminant transport     analytical solution     dispersion and advection     porous media     type    

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Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3,   Pages 179-184 doi: 10.15302/J-FASE-2014020

Abstract: In this study, we cloned the gene into a human lactoferrin (hLF) bacterial artificial chromosome (BAC) construct to make a hLF-hBCHE BAC construct.Subsequently, we injected the BAC construct into pronuclei of mouse fertilized embryos and generated

Keywords： recombinant human butyrylcholinesterase (rhBCHE)     human lactoferrin bacterial artificial chromosome (hLF BAC    

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Abundance and distribution of ammonia-oxidizing archaea in Tibetan and Yunnan plateau agricultural soils of China

Kun DING,Xianghua WEN,Liang CHEN,Daishi HUANG,Fan FEI,Yuyang LI

Frontiers of Environmental Science & Engineering 2014, Volume 8, Issue 5,   Pages 693-702 doi: 10.1007/s11783-014-0635-3

Abstract: Quantitative PCR (q-PCR) and clone library targeting on gene were used to quantify the abundances of

Keywords： ammonia-oxidizing archaea     ammonia-oxidizing bacteria     quantitative PCR     clone library     plateau    

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Effects of cotton straw amendment on soil fertility and microbial communities

Wuren HUANG, Zhihui BAI, Daniel HOEFEL, Qing HU, Xin LV, Guoqiang ZHUANG, Shengjun XU, Hongyan QI, Hongxun ZHANG

Frontiers of Environmental Science & Engineering 2012, Volume 6, Issue 3,   Pages 336-349 doi: 10.1007/s11783-011-0337-z

Abstract: metabolic activity, and markedly changed the structure of microbial community. 16S rRNA gene clone library

Keywords： cotton straw     soil amendments     microbial diversity     Biolog     phospholipid fatty acid     16S rRNA gene clone library    

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Title Author Date Type Operation

A bacterial artificial chromosome-based physical map of

Yuhua FU,Zhiqiang XIA,Shujuan WANG,Xin CHEN,Cheng LU,Mingcheng LUO,Hongbin ZHANG,Wenquan WANG

Journal Article

Development of pretreatment protocol for DNA extraction from biofilm attached to biologic activated carbon (BAC

Shuting ZHANG, Bo WEI, Xin YU, Bing LIU, Zhuoying WU, Li GU

Journal Article

Low-cost green building practice in China: Library of Shandong Transportation College

Yingxin ZHU, Borong LIN, Bin YUAN,

Journal Article

software for land use regression modeling the spatial distribution of air pollutants using GDAL/OGR library

Xuying Ma, Ian Longley, Jennifer Salmond, Jay Gao

Journal Article

Near-infrared benzodiazoles as small molecule environmentally-sensitive fluorophores

Journal Article

Intelligent Model Library for Nonferrous Metal Industry: Construction Method and Application

Yang Chunhua, Liu Yishun, Huang Keke, Sun Bei, Li Yonggang, Chen Xiaofang, Gui Weihua

Journal Article

Construction, characterization and application of a genome-wide promoter library in

Ting Yuan,Yakun Guo,Junkai Dong,Tianyi Li,Tong Zhou,Kaiwen Sun,Mei Zhang,Qingyu Wu,Zhen Xie,Yizhi Cai,Limin Cao,Junbiao Dai

Journal Article

Construction of a universal recombinant expression vector that regulates the expression of human lysozyme in milk

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Journal Article

Impact of preozonation on the bioactivity and biodiversity of subsequent biofilters under low temperature conditions—A pilot study

Jiaxuan YANG, Jun MA, Dan SONG, Xuedong ZHAI, Xiujuan KONG

Journal Article

High-Affinity Decoy PD-1 Mutant Screened from an Epitope-Specific Cell Library

Hao Liu, Chunxia Qiao, Naijing Hu, Zhihong Wang, Jing Wang, Jiannan Feng, Beifen Shen, Yuanfang Ma, Longlong Luo

Journal Article

Evaluate HAA removal in biologically active carbon filters using the ICR database

Hsin-hsin TUNG, Yuefeng F. XIE

Journal Article

Analytical solutions of three-dimensional contaminant transport in uniform flow field in porous media: A library

Hongtao WANG, Huayong WU

Journal Article

Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Journal Article

Abundance and distribution of ammonia-oxidizing archaea in Tibetan and Yunnan plateau agricultural soils of China

Kun DING,Xianghua WEN,Liang CHEN,Daishi HUANG,Fan FEI,Yuyang LI

Journal Article

Effects of cotton straw amendment on soil fertility and microbial communities

Wuren HUANG, Zhihui BAI, Daniel HOEFEL, Qing HU, Xin LV, Guoqiang ZHUANG, Shengjun XU, Hongyan QI, Hongxun ZHANG

Journal Article